Table 2 Results of screening for PbOVby conventional and real-time PCR.

From: First detection of bat-borne Issyk-Kul virus in Europe

Sample identification

Organs used for Virome

Results PbOV

Bat ID

Species

Lu

Li

He

Ki

Sp

Br

SG

In

cPCR + Sanger

qPCR results (Ct)a

E 108/09

Eptesicus nilssonii

1

 

1

 

1

1

 

1

Sp, Lu

Lu 38. Sp 38, In 38

E 138/09

Eptesicus nilssonii

1

1

  

1

  

1

Li, Sp, In

Li 38, Sp 37, In 38

E 139/09

Eptesicus nilssonii

1

1

  

1

   

Sp, Lu

–

E 141/09

Eptesicus nilssonii

1

1

  

1

   

N

n/d

E 143/09

Eptesicus nilssonii

1

1

  

1

   

Br, In, He

Sp 37, Br 40

E 145/09

Eptesicus nilssonii

1

       

N

n/d

E 147/09

Eptesicus nilssonii

1

   

1

  

1

Lu

–

E 148/09

Eptesicus nilssonii

1

   

1

   

Sp

–

E 193/07

Eptesicus nilssonii

1

   

1

   

N

n/d

E 194/07

Eptesicus nilssonii

1

      

1

Lu, Li, Sp

Br 29, Lu 25, Li 19, Sp 37

E 196/07

Eptesicus nilssonii

1

       

Li, Sp

Lu 36, Sp 37

E 202/07

Eptesicus nilssonii

    

1

   

Li, Lu

Sp 38

  1. Lu lungs, Li liver, He heart, Ki kidney, Sp spleen, Br brain, SG salivary glands, In intestine, cPCR conventional PCR, qPCR quantitative PCR.
  2. aqPCR values represent the copies/µl: ranging from Ct 40 ~ 1 copy to Ct 19 ~ 10 million copies.
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