Figure 2

Treg and Tr1 cells increase during infection with S. ratti. BALB/c, F1 and C57BL/6 mice were left naïve (day 0) or infected with 2000 iL3. Six days later mes LN were isolated and stained with a panel for regulatory receptors. Frequencies of (a) Foxp3⁺ cells in CD4⁺ T cells and (b) Tr1 cells in Foxp3⁻ CD4⁺ T cells are shown. Numbers of mes LN (c), CD4⁺ T cells (d), Foxp3⁺ Treg (e), and CD49b⁺ LAG-3⁺ Tr1 cells (f). Each symbol represents values from a single mouse. Shown are combined results from 2–3 experiments (BALB/c day 0 p.i.: n = 3, day 6 p.i.: n = 14; F1 day 0 p.i.: n = 5, day 6 p.i.: n = 13; C57BL/6 day 0 p.i.: n = 4 mice, day 6 p.i.: n = 8). Data were analyzed by an unpaired t-test. Asterisks indicate significant differences of the mean (*p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.001).