Figure 4
From: Elucidating different pattern of immunoregulation in BALB/c and C57BL/6 mice and their F1 progeny
Increased expression of distinct checkpoint molecules by Treg from C57BL/6 mice. BALB/c, F1 and C57BL/6 mice were left naïve (day 0) or infected with 2000 iL3. Six days later mes LN were isolated and stained with a panel for regulatory receptors (a–f) or ILT3 (g). Statistical analysis showing the expression of CTLA-4 (a), BTLAhigh (b), CD49b (c), VISTA (d), LAG-3 (e), PD-1 (f), and ILT3 (g) by Treg. The gating strategy is shown in the Supplementary Figs. S1 and S2. Each Symbol represents one mouse. Shown are combined results from 2 to 3 experiments (a-f: BALB/c day 0 p.i.: n = 3, day 6 p.i.: n = 14; F1 day 0 p.i.: n = 5, day 6 p.i.: n = 13; C57BL/6 day 0 p.i.: n = 4 mice, day 6 p.i.: n = 8; g: BALB/c day 0 p.i.: n = 3 mice; F1 day 0 p.i.: n = 5 mice; C57BL/6 day 0 p.i.: n = 5 mice; n = 8 mice for all infected mouse strains). Data (a–f) were analyzed by 1-way ANOVA with Tukey's multiple comparisons test. Differences in the ILT3 expression (g) were analyzed by Kruskal–Wallis test. Black asterisks are shown for naïve and infected mice and indicate significant differences of the mean (*p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.001, ****p ≤ 0.0001, ns = non significant). If in a graph only a large bar with ns is depicted, no statistical differences between the three genotypes exist at this time point. Grey asterisks indicate significant differences between day 0 and day 6 p.i. from one murine genotype.
