Figure 7
From: Elucidating different pattern of immunoregulation in BALB/c and C57BL/6 mice and their F1 progeny
Depletion of Treg in F1 mice results in improved expulsion of S. ratti from the intestine. BALB/c DEREG, F1 DEREG and C57BL/6 DEREG mice and their littermates were infected with 2000 iL3 s.c. in the footpad. DEREG mice (− Treg) and their non-transgenic littermates (+ Treg) were treated with 0.5 µg DT one day prior to S. ratti infection and on the 2 following days. (a) Parasitic adults in the small intestine were counted at day 6. Shown are combined results from 3 experiments (BALB/c + /− Treg: n = 13; F1 + Treg: n = 9, F1 − Treg: n = 11; C57BL/6 + Treg: n = 11, C57BL/6 − Treg: n = 12). (b) Concentration of mMCPT-1 in the serum of day 5 infected mice. Each symbol represents the worm burden and the amount of mMCPT-1 from a single mouse. Horizontal lines represent the mean. Shown are combined results from 3 different experiments with n = 10 mice per group. Data were analyzed by unpaired t-test comparing mice with and without Treg from one genotype. Asterisks indicate significant differences of the mean (*p ≤ 0.05, ***p ≤ 0.001, ****p ≤ 0.0001).
