Figure 3
B. subtilis ΔglpD and Δaro mutants have increased resistance to PGLYRP4-induced killing (a), lower amounts of menaquinone (MK) (b), exogenous MK increases their sensitivity to PGLYRP4-induced killing (c), and MK synthesis inhibitor DPA inhibits PGLYRP4-induced killing, which is restored by addition of exogenous MK (d). The results are means ± SEM of the numbers of bacteria (CFU) expressed as percent of initial inoculum (100%) (a,c,d) or of amounts of MK7/culture (b) and are from 4 to 6 (a,c,d) or 3 (b) experiments (biological replicates). B. subtilis 6633 was used in (d). ^P < 0.05, ^^P < 0.001 mutant vs parental strain; NS not significant (P > 0.05); *P < 0.05, **P < 0.001 as indicated by brackets.
