Table 2 Antioxidants potential activities of callus cultures of Fagonia indica elicited with UV-C radiations (0–10.8 kJ/m2) and grown in different photoperiod regimes using FRAP and ABTS cell free in vitro assays. Antioxidant capacity of samples was expressed in terms of TEAC (Trolox C equivalent antioxidant capacity) per mg of DW.

From: UV-C mediated accumulation of pharmacologically significant phytochemicals under light regimes in in vitro culture of Fagonia indica (L.)

 

FRAP

ABTS

UV + photoperiod (16L/8D h)

UV (10.8 kJ/m2)

162.1 ± 0.6 f.

390.9 ± 0.1 h

UV (9.0 kJ/m2)

168.2 ± 0.4 e

474.4 ± 0.2 b

UV (7.2 kJ/m2)

177.7 ± 0.6 c

478.2 ± 0.6 b

UV (5.4 kJ/m2)

182.3 ± 0.6 b

489.1 ± 0.3 a

UV 3.6 kJ/m2)

179.8 ± 1.1 b

466.7 ± 0.4 c

UV (1.8 kJ/m2)

152.2 ± 0.6 g

425.2 ± 0.4 f.

Control

168.2 ± 0.6 e

412.1 ± 0.4 g

UV + cont. light (24 h)

UV (10.8 kJ/m2)

158.5 ± 0.2 f.

456.4 ± 0.2 d

UV (9.0 kJ/m2)

160.2 ± 0.3 f.

472.2 ± 0.2 bc

UV (7.2 kJ/m2)

168.5 ± 0.4 e

486.1 ± 0.3 a

UV 5.4 kJ/m2)

155.4 ± 0.4 g

418.7 ± 0.1 f.

UV (3.6 kJ/m2)

174.3 ± 0.2 d

439.2 ± 0.4 e

UV (1.8 kJ/m2)

135.1 ± 0.4 i

304.6 ± 0.3 l

Control

162.4 ± 0.7 f.

384.9 ± 0.8 i

UV + Dark (24 h)

UV (10.8 kJ/m2)

120.8 ± 0.3 k

380.8 ± 0.4 i

UV (9.0 kJ/m2)

125.0 ± 0.3 j

469.0 ± 0.3 c

UV (7.2 kJ/m2)

194.5 ± 0.2 a

444.7 ± 0.2 e

UV (5.4 kJ/m2)

126.5 ± 0.2 j

397.8 ± 0.4 h

UV (3.6 kJ/m2)

149.3 ± 0.2 g

442.3 ± 0.9 e

UV (1.8 kJ/m2)

109.2 ± 0.4 l

358.3 ± 0.2 j

Control

141.5 ± 0.4 h

341.8 ± 0.2 k

  1. Values are the mean of three triplicates ± standard error (SE). Different letters represent significant differences between the various experimental conditions (p < 0.05).