Figure 2

Backscatter data from three different microplate cultivation experiments of S. pasteurii in supplemented chemically defined media to determine substrate growth limitations. Illustrated are backscatter data from different growth curves after adding grouped or single components. The reference data corresponds to cultivation in chemically defined medium (Table 1). The cultivation experiments a, b and c were stopped during stationary phase resulting in different total cultivation durations. Average pH of all cultures: 9.29 ± 0.12. Culture conditions: 48-well Flower Well Plate, filling volume 800 \(\upmu\)L, shaking frequency 1200 rpm, shaking diameter 3 mm and temperature 30 \(^\circ\)C. Here, arithmetic mean values derived from biological replicates (N = 3) are shown. The error bands depict the standard deviation.