Figure 5 | Scientific Reports

Figure 5

From: Revealing nutritional requirements of MICP-relevant Sporosarcina pasteurii DSM33 for growth improvement in chemically defined and complex media

Figure 5

Oxygen transfer rate (OTR) and backscatter of shake flask cultivations of S. pasteurii in different media. Backscatter data was generated using the Cell Growth Quantifier system and cannot be compared to backscatter data from microplate cultivation. Cultivation was carried out in (a) chemically defined medium, (b) improved chemically defined medium (c) improved YE medium. Cells were harvested when the OTR decreased to near 0 mmol/L/h. Average pH of all cultures: 9.26 ± 0.09. Culture conditions: 250 mL shake flasks, filling volume 10 mL, shaking frequency 300 rpm, shaking diameter 50 mm and temperature 30 \(^\circ\)C. Due to the experimental setup, no biological replicates were obtained simultaneously. However, the experiment was repeated with the same results (see Supplementary Figure S10).

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