Figure 2
From: Increasing AFM colloidal probe accuracy by optical tweezers
Panels A–C: Calibration curves for determination of the trap stiffness presented as a dependence of the laser beam speed on the sphere displacement. The laser beam power was in the range of: (A) 10–40%, (B) 45–75%, and (C) 80–100%. Panel D: The reconstruction of the epoxy glue distribution on the cantilever: (a) fluorescence microscopy image, and (b) three-dimensional (3D) reconstruction. 3D reconstructions were assembled with a fluorescence microscopy to visualize the distribution of adhesive layers on the cantilever surface. The adhesive layer was marked with a green color. Images were captured on the confocal setup shown in Fig. 1C and then deconvolved using the DeconvolutionLab software40,41,42.
