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Figure 1

From: Enhanced oncolytic adenoviral production by downregulation of death-domain associated protein and overexpression of precursor terminal protein

Figure 1Figure 1

Enhanced adenovirus production by oncolytic adenovirus armed with Daxx shRNA. (A) Scheme of E1B55K-deleted oncolytic adenovirus armed with scrambled RNA (Ad-3484-NC) or Daxx shRNA (Ad-3484-shDaxx). (B) Expression of Daxx protein after infection with Ad-3484-NC or Ad-3484-shDaxx at the indicated MOIs for 48 h in DU145 cells. (C) Endogenous Daxx protein levels in various human cancer cells. (D) Plaque-forming unit (PFU) titration assays of adenovirus produced by each human cancer cell line following Ad-3484-NC or Ad-3484-shDaxx infection at MOI 50 for 48 h with exchanged media after PBS washing. Error bars represent standard errors from three independent experiments. P values less than 0.05 were considered statistically significant (*P < 0.05; **P < 0.01; ***P < 0.001). (E) Crystal violet oncolytic assay of DU145 (left), U251N (middle), and A549 cells (right) following Ad-NC, Ad-shDaxx, Ad-3484-NC, or Ad-3484-shDaxx infections at MOIs between 0 and 10 for 72 h. All cells remaining on plates were fixed with 4% paraformaldehyde and stained with 0.05% crystal violet.

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