Figure 2 | Scientific Reports

Figure 2

From: Cytotoxic Escherichia coli strains encoding colibactin, cytotoxic necrotizing factor, and cytolethal distending toxin colonize laboratory common marmosets (Callithrix jacchus)

Figure 2

PCR amplification of cyclomodulin genes in representative E. coli isolates from marmosets. (a) Amplification of clbQ gene from pks island. (b) Multiplex PCR amplification was used to detect both chromosomal and plasmid cnf genes. (c) Multiplex PCR amplification was used to detect all known variants of the cdtB gene. PCR gels: lane 1,1-kb ladder; lane 2, blank; lane 3, negative control; lane 4, positive control; lanes 5 through 16, representative E. coli isolates from marmosets. Both cdt-positive isolates shown (lanes 13 and 14) are from the same animal. (d) Distribution of cyclomodulin genotypes of E. coli isolated from marmosets according to colony. Colony B had a significantly different distribution than either of the other two colonies. *** p < 0.001, Fisher’s Exact Test. Unedited images of gels are shown in Supplementary Figure S6.

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