Figure 2
Effects of the mutation of the cdgD gene on biofilm and EPS production determined in the A. brasilense WT and derivative strains. (a) Determination of biofilm formation in NFB* medium supplemented with KNO3 via the quantification of CV/mg protein values. (b) Determination of biofilm formation in NFB* supplemented with NH4Cl medium via the quantification of CV/mg protein values. (c,d) Determination of EPS production in the media described above via the quantification of CR/mg protein values in a staining assay. Wild type (Sp245), isogenic mutant (12-A), complemented mutant (C-56A), and empty vector mutant (C-40A). Error bars represent the standard deviations of three biological replicates, and statistically significant differences are indicated at *P < 0.05 according to Student’s t-test by SigmaPlot (Systat Software, San Jose, CA).
