Figure 1
From: Acute systemic loss of Mad2 leads to intestinal atrophy in adult mice
Inactivation of Mad2 knockout leads to rapid atrophy in jejunum/ileum. (A) Relative weight compared to starting weight over time. Plotted average weight with standard error of the mean (SEM), n = 4 per condition. Two-sided t test was used to test difference between each genetic group and the control, P value per timepoint and per genotype displayed. (B) Representative images of jejunum of control and Mad2; p53 compound knockout mice. H&E staining, scale bar is 60 μm. (C) Representative images of villi and crypts of the jejunum showing normal jejunum (top panel) and Mad2f/f; p53f/f; Cre-ERT2 jejunum with atrophied villi and atypical cells (bottom panel). H&E staining, scale bar 100 μm. Lines and numbers represent the crypt and villus length, and are quantified for multiple images in (E). (D) Representative images of villi and crypts of the jejunum per genotype. H&E staining, scale bar is 60 μm. Black arrows point to apoptotic cells. (E) Scatter plot of villus length and apoptotic cells per high power field (HPF) image per genotype.
