Figure 2
From: Acute systemic loss of Mad2 leads to intestinal atrophy in adult mice
Acute Mad2 loss leads to apoptosis and mitotic abnormalities in intestine. (A) Representative images of control and Mad2f/f; p53f/f; Cre-ERT2 jejunum/ileum immuno-stained for cleaved Caspase3. Insets show zoom-in of intestinal crypts. (B) Quantification of fraction of Caspase 3-cleaved cells in intestine for each assessed mouse. Minimum of six representative images per mouse, and minimum of 100 cells quantified per image. P value is shown for a t test between the three genotypes. Error bars indicate the SEM. (C) Representative images of a normal mitosis (upper panel) and abnormal mitosis (bottom panel) in the intestinal crypt. H&E staining of mouse jejunum. (D) Quantification of the observed mitotic abnormalities per genotype assessed from tissue samples. Chi-squared test comparing cumulative mitotic abnormalities to normal mitosis per condition. “n” refers to the total number of mitotic events quantified per genotype.
