Figure 3
From: Acute systemic loss of Mad2 leads to intestinal atrophy in adult mice
Acute Mad2 loss leads to a modest phenotype in spleen within the first 4 days (A) Mad2f/f; p53f/f; Cre-ERT2 spleen showing cellular atypia in the red pulp, scale bar 20 μm, HE staining, magnification ×400. (B) Quantification of spleen abnormalities between genotypes, n = 4 per genotype. P values refer to Wilcox rank‐sum test. (C) Representative images of mouse spleen immuno-stained for cleaved Caspase3. (D) Zoom-in of an atypical cell positive for cleaved Caspase 3. (E) Quantification of fraction of Caspase 3-cleaved cells in spleen for each assessed mouse. Minimum of six representative images per mouse, and minimum of 100 cells quantified per image. P value is shown for a t test between the three genotypes, P < 0.01 was considered significant. Error bars indicate the SEM. (F) Representative images of a normal mitosis (upper panel) and abnormal mitosis (bottom panel) in the intestinal crypt. H&E staining of mouse jejunum. (G) Quantification of the observed mitotic abnormalities per genotype assessed from tissue samples. Chi-squared test comparing cumulative mitotic abnormalities to normal mitosis per condition. “n” refers to the total number of mitotic events quantified per genotype.
