Table 3 Detailed information of mutations in thyA, ribD, and folC and PAS/SMX DST results of the 95 clinical isolates. ID identification, NO no mutations present, MIC minimal inhibitory concentration.
From: Mutations of folC cause increased susceptibility to sulfamethoxazole in Mycobacterium tuberculosis
Sample information | MIC (L–J) | MIC (7H10) | ||||
|---|---|---|---|---|---|---|
Mutational gene | Sample ID | Nucleotide alteration | Amino acid alteration | PAS (μg/ml) | PAS (μg/ml) | SMX (μg/ml) |
Control | H37Rv | NO | < 1 | < 0.1 | 50 | |
K6457 | NO | < 1 | < 0.1 | 50 | ||
K8497 | NO | < 1 | 0.2 | 50 | ||
K6533 | NO | < 1 | 0.2 | 50 | ||
K5970 | NO | < 1 | < 1 | 50 | ||
K8313 | NO | < 1 | 0.2 | 50 | ||
K3270 | NO | > 1 | > 16 | 50 | ||
E260 | NO | > 1 | > 16 | 50 | ||
thyA | K8902 | 64A>C | Thr22Pro | > 1 | > 16 | 50 |
K6328 | 127C>T | Pro43Ser | > 1 | > 16 | 50 | |
K6487 | 290A>G | Gln97Arg | > 1 | > 16 | 50 | |
K6407 | 329A>G | Asp110Gly | > 1 | 8 | 50 | |
K3543 | 399G>A | Trp133Stop | > 1 | > 16 | 50 | |
K4287 | 440A>G | His147Arg | > 1 | > 16 | 50 | |
K5819 | 440A>G | His147Arg | > 1 | > 16 | 100 | |
E945 | 505G>A | Asp169Asn | > 1 | > 16 | 50 | |
F461 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
K5999 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
K3229 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
K4208 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
K6079 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
E531 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
F330 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
E940 | 704G>C | Arg235Pro | > 1 | > 16 | > 100 | |
F462 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
K9133 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
E576 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
K2481 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
K8646 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
K3211 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
K5514 | 704G>C | Arg235Pro | > 1 | > 16 | 50 | |
ribD | K5874 | − 12G>A | > 1 | 16 | 50 | |
K8941 | − 12G>A | > 1 | 4 | 100 | ||
K7234 | − 12G>A | > 1 | > 2 | 50 | ||
K8433 | − 12G>A | > 1 | > 2 | 50 | ||
E944 | − 12G>A | > 1 | 8 | 50 | ||
K2614 | − 12G>A | > 1 | > 2 | 50 | ||
K4239 | − 12G>A | > 1 | > 2 | 50 | ||
K4160 | 745G>A | Gly249Ser | > 1 | > 2 | 50 | |
K3491 | 745G>A | Gly249Ser | > 1 | > 2 | 50 | |
K4854 | 745G>A | Gly249Ser | > 1 | > 2 | 50 | |
thyA; ribD | F241 | 704G>T; 273G>A | Arg235Leu; Glu91Glu | > 1 | 4 | 100 |
folC | K6599 | 118G>A | Glu40Lys | > 1 | 16 | 50 |
KA792 | 119A>G | Glu40Gly | > 1 | > 2 | 10 | |
K3283 | 119A>G | Glu40Gly | > 1 | 2 | 20 | |
E958 | 119A>G | Glu40Gly | > 1 | 16 | 10 | |
F508 | 128T>G | Ile43Ser | > 1 | > 2 | < 5 | |
K6640 | 128T>G | Ile43Ser | > 1 | > 2 | 5 | |
E941 | 128T>C | Ile43Thr | > 1 | > 16 | 50 | |
K4279 | 128T>C | Ile43Thr | > 1 | > 16 | 50 | |
K6545 | 128T>C | Ile43Thr | > 1 | > 16 | 50 | |
K8315 | 128T>C | Ile43Thr | > 1 | > 16 | 50 | |
E578 | 128T>C | Ile43Thr | > 1 | > 16 | 50 | |
K8301 | 128T>C | Ile43Thr | > 1 | > 16 | 20 | |
K3361 | 128T> C | Ile43Thr | > 1 | > 16 | 20 | |
E903 | 128T>C | Ile43Thr | > 1 | > 16 | 20 | |
K2543 | 128T>C | Ile43Thr | > 1 | > 16 | 10 | |
K4913 | 128T>C | Ile43Thr | > 1 | 4 | 20 | |
K7601 | 128T>C | Ile43Thr | > 1 | 2 | < 5 | |
K4385 | 128T>C | Ile43Thr | > 1 | 16 | 20 | |
F122 | 128T>C | Ile43Thr | > 1 | > 16 | 50 | |
K7678 | 128T>C | Ile43Thr | > 1 | 16 | 20 | |
K4603 | 128T>C | Ile43Thr | > 1 | 16 | 10 | |
E930 | 128T>C | Ile43Thr | > 1 | > 16 | 50 | |
K3183 | 128T>C | Ile43Thr | > 1 | > 2 | 20 | |
K6361 | 128T>C | Ile43Thr | > 1 | > 2 | 20 | |
KA387 | 128T>C | Ile43Thr | > 1 | > 2 | 20 | |
KA045 | 128T>C | Ile43Thr | > 1 | > 2 | 20 | |
KA156 | 128T>C | Ile43Thr | > 1 | > 2 | 10 | |
KA636 | 128T>C | Ile43Thr | > 1 | > 2 | 20 | |
K7917 | 128T>C | Ile43Thr | > 1 | > 2 | 20 | |
K7040 | 128T>C | Ile43Thr | > 1 | 8 | 20 | |
K7805 | 128T>C | Ile43Thr | > 1 | 4 | 5 | |
K7979 | 128T>C | Ile43Thr | > 1 | > 16 | 20 | |
F036 | 128T>C | Ile43Thr | > 1 | 8 | 5 | |
K3459 | 128T>C | Ile43Thr | > 1 | > 16 | 20 | |
K5236 | 128T>C | Ile43Thr | > 1 | 8 | 10 | |
K4354 | 128T>C | Ile43Thr | > 1 | > 16 | 50 | |
K5366 | 128T>C | Ile43Thr | > 1 | > 16 | 20 | |
E285 | 127A>G; 128T>C; 129C>T | Ile43Val; Ile43Thr; Ile43Ile | > 1 | > 16 | 50 | |
K4303 | 131A>G; 136A>C | Asp44Gly; Ser46Arg | > 1 | 16 | 50 | |
F970 | 145C>T | Arg49Trp | > 1 | > 2 | 20 | |
F899 | 146G>A | Arg49Gln | > 1 | 2 | 20 | |
F077 | 448A>G | Ser150Gly | > 1 | 16 | 20 | |
K5303 | 448A>G | Ser150Gly | > 1 | 4 | 20 | |
F375 | 448A>G | Ser150Gly | > 1 | > 2 | 10 | |
KA187 | 448A>G | Ser150Gly | > 1 | > 2 | 10 | |
K6842 | 448A>G | Ser150Gly | > 1 | > 16 | 20 | |
K6722 | 458A>C | Glu153Ala | > 1 | > 2 | 20 | |
KA779 | 458A>C | Glu153Ala | > 1 | > 2 | < 5 | |
KA712 | 458A>C | Glu153Ala | > 1 | > 2 | 10 | |
KA391 | 458A>C | Glu153Ala | > 1 | > 2 | 5 | |
KA440 | 458A>C | Glu153Ala | > 1 | > 2 | 10 | |
G216 | 458A>G | Glu153Gly | > 1 | > 2 | 5 | |
K9669 | 529_530insG | Frame shift | > 1 | 16 | 20 | |
