Figure 12
From: NSC348884 cytotoxicity is not mediated by inhibition of nucleophosmin oligomerization
NPM oligomerization after NSC348884 treatment in living cells. (a) FLIM-FRET analysis of the eGFP-fluorescence lifetime (τ) after 2 h of 10 µM NSC348884 action on cells co-transfected with red and green variants of NPMwt (upper row) or NPMmut (lower row). White numbers: fluorescence lifetime measured in individual cells. Dashed circles: region of mRFP1-photobleaching. Simultaneous cell morphology screening by DIC documents cell rounding induced by NSC348884. (b) Statistical evaluation of τ values before (control) and after (bleached) the mRFP1 photobleaching in intact (black bar) and NSC348884-treated (grey bar) cells. Student´s t-test of “control” versus “bleached” values: ****p < 0.0001, ***p < 0.001,*p < 0.05.
