Figure 2
From: NSC348884 cytotoxicity is not mediated by inhibition of nucleophosmin oligomerization
Effect of C21 mutations on the stability of NPM oligomers. Native (left) and semi-native (right) PAGE analysis of lysates from untransfected (U) 293T cells or cells transfected with NPMwt (W), C21A (A), C21F (F) or NPMmut (M). (a) Stability of oligomers formed by various G_C21 constructs is affected by the reducing PAGE conditions. (b) Position of bands and oligomer stability depend on the fluorescent label used (G or R: eGFP- or mRFP1-labeled proteins). The figures show representative examples from repeated experiments.
