Figure 1
From: The serine protease HtrA plays a key role in heat-induced dispersal of pneumococcal biofilms
Schematic overview of the model system used in this study. Biofilms were formed in flat bottom wells over 72 h in CDM at 34 °C, with change of medium every 12 h. Biofilms were washed twice in CDM and then incubated in fresh medium at 34 °C (control; upper panel) or at 38.5 °C (lower panel) for 4 h. Supernatants containing released bacteria from control (34 °C) or heat-exposed (38.5 °C) biofilms were then collected and enumerated by viable plate counts. The remaining biofilms were resuspended and collected. Supernatant and biofilm fractions were saved for gene expression analysis. The example shown is for heat-induced biofilm dispersal. Similar methods were used for inhibitor experiments and exogenous protease experiments.
