Table 3 Kinetic parameters for proteolysis of V1-5GAG by ADAMTS-5 variants.

From: Exosite inhibition of ADAMTS-5 by a glycoconjugated arylsulfonamide

ADAMTS-5 variant

kcat/Km

105 M−1 s−1

Fold reduction

MDTCS

32.3 ± 7.64

–

K532A/K533A

6.75 ± 2.22*

4.8

K532Q/K533Q

4.01 ± 0.49*

8.0

K533A

12.5 ± 2.75*

2.6

K533H

15.6 ± 4.17

2.1

  1. Values were determined by time course experiments at 50 nM V1-5GAG substrate concentration. Results are expressed as mean ± SEM. *p < 0.05, compared to ADAMTS-5 MDTCS according to Mann–Whitney tests (n = 3).
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