Table 1 Names and sequences of primers used in this study. Note: The sequences underlined are the sequence of the T7 promoter.
Primer name | Primer sequence (from 5′ to 3′) | Application |
|---|---|---|
PmC1qDC-1-F | ATGGAGACAGGTTTACCTCTTATA | CDS clone |
PmC1qDC-1-R | CTACAGTTTGTATCCAGAAAATGCT | CDS clone |
NUP | GCAGTGGTATCAACGCAGAGT | RACE |
UPM-short | CTAATACGACTCACTATAGGGC | RACE |
UPM-long | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | RACE |
PmC1qDC-1-5′-outer | CACTGAAAAATGTCCCTGCCATCTTAT | RACE |
PmC1qDC-1–5′-inner | CGTAAGTATGACCCGAAAAGGATG | RACE |
PmC1qDC-1-3′-outer | TGATGTTGGACGGGAATGTGAAAG | RACE |
PmC1qDC-1-3′-inner | ACATAAACAAGGAAGAGTAAATGCTGGGAC | RACE |
PmC1qDC-1-qPCR-F | GAAAGTCTGTAAACCTGGCACC | qRT-PCR |
PmC1qDC-1-qPCR-R | ACATTCCCGTCCAACATCAA | qRT-PCR |
GAPDH-F | GCAGATGGTGCCGAGTATGT | qRT-PCR |
GAPDH-R | CGTTGATTATCTTGGCGAGTG | qRT-PCR |
β-Actin-F | CGGTACCACCATGTTCTCAG | qRT-PCR |
β-Actin-R | GACCGGATTCATCGTATTCC | qRT-PCR |
PmC1qDC-1-T7-F | ACTCACTAATACGACTCACTATAGGGATGGAGACAGGTTTACCTCTTATA | RNAi |
PmC1qDC-1-T7-R | ACTCACTAATACGACTCACTATAGGGCTACAGTTTGTATCCAGAAAATGCT | RNAi |
ISH576.501-F | CCTTTTCGGGTCATACTTACG | FISH |
ISH576.501-R | ACTCACTAATACGACTCACTATAGGGCCAGCATTTACTCTTCCTTGTTTA | FISH |
PmC1qDC-1-XhoI | TTTTCAGGGTCTCGGATCCCTCGAGGCTTTTTCCTTGAAATACGA | Subclone |
PmC1qDC-1-EcoRI | GGTGGTGGTGGTGGGTCTCGAATTCAAGGCTCACAGCAGAGAAGT | Subclone |
