Table 1 Information of 19 sets of RT-LAMP primers for the detection of SARS-CoV-2.
Primer sets | Primer name | Primer sequence (5′-3′) | Target gene | Genomic location (nt) | Sensitivity (or LOD) | Refs |
|---|---|---|---|---|---|---|
S1 | F3 | CTGCACCTCATGGTCATGTT | orf1ab | 498–711 | 1200 copies/25 μL reaction | |
B3 | AGCTCGTCGCCTAAGTCAA | |||||
FIP | GAGGGACAAGGACACCAAGTGTATGGTTGAGCTGGTAGCAGA | |||||
BIP | CCAGTGGCTTACCGCAAGGTTTTAGATCGGCGCCGTAAC | |||||
LF | CCGTACTGAATGCCTTCGAGT | |||||
LB | TTCGTAAGAACGGTAATAAAGGAGC | |||||
S2 | F3 | TCCAGATGAGGATGAAGAAGA | orf1ab | 3043–3331 | 1.02 fg/25 μL reaction | |
B3 | AGTCTGAACAACTGGTGTAAG | |||||
FIP | AGAGCAGCAGAAGTGGCACAGGTGATTGTGAAGAAGAAGAG | |||||
BIP | TCAACCTGAAGAAGAGCAAGAACTGATTGTCCTCACTGCC | |||||
LF | CTCATATTGAGTTGATGGCTCA | |||||
LB | ACAAACTGTTGGTCAACAAGAC | |||||
S3 | F3 | GGAATTTGGTGCCACTTC | orf1ab | 3145–3345 | 100 copies/15 μL reaction | |
B3 | CTATTCACTTCAATAGTCTGAACA | |||||
FIP | CTTGTTGACCAACAGTTTGTTGACTTCAACCTGAAGAAGAGCAA | |||||
BIP | CGGCAGTGAGGACAATCAGACACTGGTGTAAGTTCCATCTC | |||||
LF | ATCATCATCTAACCAATCTTCTTC | |||||
LB | TCAAACAATTGTTGAGGTTCAACC | |||||
S4 | F3 | TGCAACTAATAAAGCCACG | orf1ab (Nsp3) | 6253–6446 | 100 copies/15 μL reaction | |
B3 | CGTCTTTCTGTATGGTAGGATT | |||||
FIP | TCTGACTTCAGTACATCAAACGAATAAATACCTGGTGTATACGTTGTC | |||||
BIP | GACGCGCAGGGAATGGATAATTCCACTACTTCTTCAGAGACT | |||||
LF | TGTTTCAACTGGTTTTGTGCTCCA | |||||
LB | TCTTGCCTGCGAAGATCTAAAAC | |||||
S5 | F3 | TGCTTCAGTCAGCTGATG | orf1ab | 13,434–13,636 | 7 copies/10 µL reaction | |
B3 | TTAAATTGTCATCTTCGTCCTT | |||||
FIP | TCAGTACTAGTGCCTGTGCCCACAATCGTTTTTAAACGGGT | |||||
BIP | TCGTATACAGGGCTTTTGACATCTA TCTTGGAAGCGACAACAA | |||||
LF | CTGCACTTACACCGCAA | |||||
LB | GTAGCTGGTTTTGCTAAATTCC | |||||
S6 | F3 | GGTATGATTTTGTAGAAAACCCA | orf1ab | 13,925–14,140 | 20 copies/25 µL reaction | |
B3 | CAACAGGAACTCCACTACC | |||||
FIP | GGCATCACAGAATTGTACTGTTTTTGCGTATACGCCAACTTAGG | |||||
BIP | AATGCTGGTATTGTTGGTGTACTGAGGTTTGTATGAAATCACCGAA | |||||
LF | AACAAAGCTTGGCGTACACGTTCA | |||||
S7 | F3 | GTTACGATGGTGGCTGTA | orf1ab | 14,885–15,081 | 5 copies/25 µL reaction | |
B3 | GGCATACTTAAGATTCATTTGAG | |||||
FIP | AGCCTTACCCCATTTATTAAATGGAGCTAACCAAGTCATCGTCAA | |||||
BIP | AATGAGTTATGAGGATCAAGATGCATTATAGTAGGGATGACATTACGT | |||||
LF | AAACCAGCTGATTTGTCTAGGTTG | |||||
S8 | F3 | AAACGTAATGTCATCCCTACT | orf1ab (RdRp) | 15,034–15,274 | 3 copies/25 μL reaction | |
B3 | GGTTTTCTACATCACTATAAACAGT | |||||
FIP | ACAGATAGAGACACCAGCTACGCTCAAATGAATCTTAAGTATGCCA | |||||
BIP | ATAGCCGCCACTAGAGGAGCCCAACCACCATAGAATTTGC | |||||
LF | GTGCGAGCTCTATTCTTTGCACTA | |||||
S9 | F3 | CCACTAGAGGAGCTACTGTA | orf1ab | 15,182–15,387 | 10 copies/20 µL reaction | |
B3 | TGACAAGCTACAACACGT | |||||
FIP | AGGTGAGGGTTTTCTACATCACTATATTGGAACAAGCAAATTCTATGG | |||||
BIP | ATGGGTTGGGATTATCCTAAATGTGTGCGAGCAAGAACAAGTG | |||||
LF | CAGTTTTTAACATGTTGTGCCAACC | |||||
LB | TAGAGCCATGCCTAACATGCT | |||||
S10 | F3 | CTGACAAAGTTTTCAGATCCTCAG | S | 21,678–21,886 | NA | |
B3 | AGTACCAAAAATCCAGCCTCTT | |||||
FIP | TCCCAGAGACATGTATAGCATGGAATCAACTCAGGACTTGTTCTTACC | |||||
BIP | TGGTACTAAGAGGTTTGATAACCCTGTTAGACTTCTCAGTGGAAGCA | |||||
LF | CCAAGTAACATTGGAAAAGAAA | |||||
LB | GTCCTACCATTTAATGATGGTGTTT | |||||
S11 | F3 | TCTATTGCCATACCCACAA | S | 23,693–23,937 | 200 copies/25 µL reaction | |
B3 | GGTGTTTTGTAAATTTGTTTGAC | |||||
FIP | CATTCAGTTGAATCACCACAAATGTGTGTTACCACAGAAATTCTACC | |||||
BIP | GTTGCAATATGGCAGTTTTTGTACATTGGGTGTTTTTGTCTTGTT | |||||
LF | ACTGATGTCTTGGTCATAGACACT | |||||
LB | TAAACCGTGCTTTAACTGGAATAGC | |||||
S12 | F3 | CCGACGACGACTACTAGC | E | 26,191–26,424 | 20 copies/10 µL reaction | |
B3 | AGAGTAAACGTAAAAAGAAGGTT | |||||
FIP | CTAGCCATCCTTACTGCGCTACTCACGTTAACAATATTGCA | |||||
BIP | ACCTGTCTCTTCCGAAACGAATTTGTAAGCACAAGCTGATG | |||||
LF | TCGATTGTGTGCGTACTGC | |||||
LB | TGAGTACATAAGTTCGTAC | |||||
S13 | F3 | AGCTGATGAGTACGAACTT | E | 26,226–26,441 | 5 copies /25 μL reaction | |
B3 | TTCAGATTTTTAACACGAGAGT | |||||
FIP | ACCACGAAAGCAAGAAAAAGAAGTATTCGTTTCGGAAGAGACAG | |||||
BIP | TTGCTAGTTACACTAGCCATCCTTAGGTTTTACAAGACTCACGT | |||||
LB | CTGCGCTTCGATTGTGTGCGT | |||||
S14 | F3 | CCAGAATGGAGAACGCAGTG | N | 28,354–28,569 | 1 copies/25 µL reaction | |
B3 | CCGTCACCACCACGAATT | |||||
FIP | AGCGGTGAACCAAGACGCAGGGCGCGATCAAAACAACG | |||||
BIP | AATTCCCTCGAGGACAAGGCGAGCTCTTCGGTAGTAGCCAA | |||||
LF | TTATTGGGTAAACCTTGGGGC | |||||
LB | TTCCAATTAACACCAATAGCAGTCC | |||||
S15 | F3 | TGGCTACTACCGAAGAGCT | N | 28,525–28,741 | 120 copies/25 μL reaction | |
B3 | TGCAGCATTGTTAGCAGGAT | |||||
FIP | TCTGGCCCAGTTCCTAGGTAGTCCAGACGAATTCGTGGTGG | |||||
BIP | AGACGGCATCATATGGGTTGCACGGGTGCCAATGTGATCT | |||||
LF | GGACTGAGATCTTTCATTTTACCGT | |||||
LB | ACTGAGGGAGCCTTGAATACA | |||||
S16 | F3 | AGATCACATTGGCACCCG | N | 28,702–28,914 | 5 copies/25 µL reaction | |
B3 | CCATTGCCAGCCATTCTAGC | |||||
FIP | TGCTCCCTTCTGCGTAGAAGCCAATGCTGCAATCGTGCTAC | |||||
BIP | GGCGGCAGTCAAGCCTCTTCCCTACTGCTGCCTGGAGTT | |||||
LF | GCAATGTTGTTCCTTGAGGAAGTT | |||||
LB | CGTAGTCGCAACAGTTAAGAAATTC | |||||
S17 | F3 | GCCAAAAGGCTTCTACGCA | N | 28,774–28,971 | NA | |
B3 | TTGCTCTCAAGCTGGTTCAA | |||||
FIP | TCCCCTACTGCTGCCTGGAGGCAGTCAAGCCTCTTCTCG | |||||
BIP | TCTCCTGCTAGAATGGCTGGCATCTGTCAAGCAGCAGCAAAG | |||||
LF | TGTTGCGACTACGTGATGAGGA | |||||
LB | ATGGCGGTGATGCTGCTCT | |||||
S18 | F3 | GCCAAAAGGCTTCTACGCA | N | 28,774–28,971 | 20 copies /25 µL reaction (118.6 copies/25 µL reaction) | |
B3 | TTGCTCTCAAGCTGGTTCAA | |||||
FIP | TCCCCTACTGCTGCCTGGAGCAGTCAAGCCTCTTCTCGTT | |||||
BIP | TCTCCTGCTAGAATGGCTGGCATCTGTCAAGCAGCAGCAAAG | |||||
LB | TGGCGGTGATGCTGCTCTT | |||||
S19 | F3 | AACACAAGCTTTCGGCAG | N | 29,083–29,311 | 20 copies/10 µL reaction | |
B3 | GAAATTTGGATCTTTGTCATCC | |||||
FIP | CGCATTGGCATGGAAGTCACTTTGATGGCACCTGTGTAG | |||||
BIP | TGCGGCCAATGTTTGTAATCAGCCAAGGAAATTTTGGGGAC | |||||
LF | TTCCTTGTCTGATTAGTTC | |||||
LB | ACCTTCGGGAACGTGGTT |
