Table 2 Comparison of primer sets developed by WHO referral labs, against the primer set obtained by the proposed approach, listed as UtrechtU-ORF3a.
From: Classification and specific primer design for accurate detection of SARS-CoV-2 using deep learning
Primer | Sequence | Frequency (%) | Specific (Yes/No) |
|---|---|---|---|
Charite-E-F | 5′-ACA GGT ACG TTA ATA GTT AAT AGC GT-3′ | 99.90 | No |
Charite-E-R | 5′-ATA TTG CAG CAG TAC GCA CAC A-3′ | 99.90 | No |
CHINA-CDC-ORF1ab-F | 5′-CCC TGT GGG TTT TAC ACT TAA-3′ | 99.90 | Yes |
CHINA-CDC-ORF1ab-R | 5′-ACG ATT GTG CAT CAG CTG A-3′ | 99.59 | Yes |
HKU-N-F | 5′-TAA TCA GAC AAG GAA CTG ATT A-3′ | 99.56 | No |
HKU-N-R | 5′-CGA AGG TGT GAC TTC CAT G-3′ | 99.58 | No |
US-CDC-N1-F | 5′-GAC CCC AAA ATC AGC GAA AT-3′ | 99.71 | Yes |
US-CDC-N1-R | 5′-TCT GGT TAC TGC CAG TTG AAT CTG-3′ | 99.57 | Yes |
US-CDC-N2-F | 5′-TTA CAA ACA TTG GCC GCA AA-3′ | 99.43 | No |
US-CDC-N2-R | 5′-GCG CGA CAT TCC GAA GAA-3′ | 99.74 | Yes |
UtrechtU-ORF3a-F | 5′-TAG CAC TCT CCA AGG GTG TTC-3′ | 99.57 | Yes |
UtrechtU-ORF3a-R | 5′-GCA AAG CCA AAG CCT CAT TA-3′ | 99.48 | Yes |
US-CDC-N3-F | 5′-GGG AGC CTT GAA TAC ACC AAA A-3′ | 99.09 | Yes |
US-CDC-N3-R | 5′-TGT AGC ACG ATT GCA GCA TTG-3′ | 99.72 | Yes |
CHINA-CDC-N-F | 5′-GGG GAA CTT CTC CTG CTA GAA T-3′ | 68.52 | Yes |
CHINA-CDC-N-R | 5′-CAG ACA TTT TGC TCT CAA GCT G-3′ | 99.20 | Yes |
