Figure 6

Quantification of neurite formation in primary hippocampal cells isolated from the hippocampus of the rat offspring prenatally exposed to BPA or vehicle control. Hippocampal cells were isolated from neonatal rat pups prenatally exposed to BPA or the vehicle control and cultured for 7 days. Images of the cells were taken using an inverted microscope. Sholl analysis (a–c) and evaluation of total neurite length (d–e), number of primary neurites and branching (f–i), and cell body area (j–k) were performed. Data are presented as the mean ± SEM. *^,#P value < 0.05. *Indicates a statistically significant difference between the BPA treatment and the vehicle control groups. ^#Indicates a statistically significant difference between males and females in the BPA group on the same day.