Table 1 Loci and primer characteristics.
Locus | Genbank (UniSTS) | Chromosome location | Physical distance (Mb) | Genetic distance (cM) | Motif | Allele range | Primer sequence (5′-3′) | Tm (℃) | Amplicon size(bp) | Dye label |
|---|---|---|---|---|---|---|---|---|---|---|
DXS6795 | G09877 | p22.11 | 23.254 | 44.24 | AAT | 9–14 | F-CATCCATCCCCTAAACCTCTCATG | 60.28 | 118–155 | FAM |
R-TTTCAGCTCCTAGGTGACTGGGA | 56.62 | |||||||||
DXS6803 | G08107 (41,731) | q21.2 | 86.318 | 99.4 | TCTA | 10–14.3 | F-GAACATATGCTACTTTATCTCGA | 56.7 | 177–218 |  |
R-GCATATAAATTTTGAATTAAAG | 57.18 | |||||||||
DXS6807 | G09662 (44,289) | p22.33 | 4.753 | 14.76 | GATA | 11–16 | F-TATGAAAAGAAAGGAAGCACCAA | 52.67 | 240–277 |  |
R-ACAGGAGATATATAGTAAAAATAC | 57.83 | |||||||||
DXS9907 | G27255 (39,433) | p21.1 | 32.01 | 55.32 | CTAT | 9–15 | F-TTCTCCCATCTCAGCCTCCTG | 54.74 | 291–337 |  |
R-ACAAAGGATGTTAAAGCATCATC | 54.43 | |||||||||
DXS7423 | 95,583 | q28 | 149.46 | 184.19 | TCCA | 14–17 | F-CACCCAGATTTCCTCCCCA | 54.54 | 347–395 |  |
R-GCCACTGGGGACATGTGAATGG | 57.88 | |||||||||
AMEL | AC002366.1 (AC013412.3) | X: p22.1–22.3 Y:p11.2 |  |  | N.A | X, Y | F-CCCTGGGCTCTGTAAAGAATA | 56.8 | 99–107 | HEX |
R-ATCAGAGCTTAAACTGGGAAGCTG | 56.97 | |||||||||
GATA172D05 | 498,601 | q23 | 113.061 | 124.36 | TAGA | 6–12 | F-TACTGTTTAACCAGTACAAAGTT | 51.99 | 124–168 |  |
R-ATAGATATAGGTATTGATATAG | 54.5 | |||||||||
DXS101 | 98,951 | q22.1 | 101.3 | 116.15 | CTT/ATT | 20–27 | F-ACAAAGATAATACACACATATTCAT | 57.75 | 179–231 |  |
R-AGTGAGCATACATACATGTATCT | 57.74 | |||||||||
DXS9902 | G27261(73,076) | p22.2 | 15.234 | 32.32 | GATA | 7–14 | F-AATGGAGTCTCTGGGTGAAGAG | 56.52 | 244–288 |  |
R-CATATAATTATTTTCTTTG | 56.65 | |||||||||
DXS7133 | G08113(50,709) | q22.3 | 108.928 | 118.18 | ATAG | 6–12 | F-ATATCTCCACTTCCAAAAGGGG | 58.65 | 320–351 |  |
R-CTGTTCACCTTTCCGAGTCTTC | 59.67 | |||||||||
DXS6810 | G09983(7699) | p11.3 | 42.804 | 75.12 | CTAT | 16–20 | F-ACCTCAGGTGATCCACCTGCCTCG | 56.16 | 365–395 |  |
R-CATAGCTTAGCAAACAGCTGTATG | 53.95 | |||||||||
GATA31E08 | G08104(15,349) | q27.1 | 140.062 | 160.54 | AGGG/AGAT | 6–13 | F-TGTTTATCATACTAGATATAGATA | 54.7 | 107–145 | TAMRA |
R-AGATACATGTATGTATGCTCACT | 58.51 | |||||||||
DXS6800 | G09609(58,372) | q13.3 | 78.567 | 97.49 | TAGA | 15–22 | F-TGAAATATTGGGGGCTGGTTCC | 55.1 | 165–207 |  |
R-CTTGCTCCTCAGCTTGCAGAGGG | 55.18 | |||||||||
DXS981 | 99,675 | q13.1 | 68.114 | 92.81 | TATC | 12–16 | F-AAGTCACCACCATATTGTTCCTTGA | 52.09 | 213–249 |  |
R-GGAAAAGAAGTAGACATACTTC | 54.02 | |||||||||
DXS10162 | NC_000023 | q11.1 | 61.8 | 90.65 | TCTT | 15–21 | F-TCACTAAGCAGTTTCATAGATAG | 63.49 | 275–345 |  |
R-GGAAAACCAGGTTATCCCCAG | 60.26 | |||||||||
DXS6809 | G08112(69,546) | q21.33 | 94.825 | 108.12 | ATCT/CTAT | 29–36 | F-TCTGGAGAATCCAATTTTGC | 53.4 | 347–404 |  |
R-GGCCTCACTTAATGGATGAAGCA | 54.78 | |||||||||
GATA165B12 | G10699(63,387) | q25 | 120.706 | 136.18 | AGAT | 8–12 | F-CTGATATACGGATAGATGATTG | 57.25 | 86–128 | ROX |
R-AACTTTTAAAGCATACACAGTG | 54.45 | |||||||||
DXS10079 | AL049564 | q12 | 66.632 | 90.82 | AGAA | 16–23 | F-AGATTGTGCCAATGCTCTCCAG | 55.18 | 133–191 |  |
R-AGGTATATTGGCCTGTAG | 59.12 | |||||||||
DXS10135 | AC003684 | p22.31 | 9.199 | 20.03 | GAAA | 15–33 | F-GCATTTGAAACTAAAGTCAAATG | 58.19 | 201–313 |  |
R-AATACCTGGATCTAGCCAAGCCTG | 55.51 | |||||||||
HPRTB | M26434 | q26.2 | 133.443 | 149.66 | AGAT | 10–16 | F-GTAGTTTCTTAAAGTGTCTC | 53.15 | 323–375 |  |
R-ATACACATCCCCATTCCTG | 54.85 |
