Figure 3 | Scientific Reports

Figure 3

From: Dissecting the contribution of O-Antigen and proteins to the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA)

Figure 3

Immunogenicity study of GMMA in mice: analysis of serum IgG content and functionality. Single sera were collected from the differently immunized groups (8 mice/group). Single sera have been analysed in ELISA using as coating antigens S. sonnei full LPS (a) or OAg-negative GMMA (b) and in SBA against OAg-positive (c) and OAg-negative (d) S. sonnei strains. Geometric means and individual values are plotted in the graphs. Mann–Whitney test was performed between the two groups immunized with OAg-positive GMMA, the two groups immunized with OAg-negative GMMA and between the groups immunized with GMMA sharing the same lipid A structure (*P < 0.05, **P < 0.01, ***P < 0.001). S. sonnei bacteria displaying (e) or not (f) the OAg have been stained with pooled sera from the study and analyzed by FACS. Mean Fluorescence Intensity (MFI) of 10,000 aquired events is reported.

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