Figure 2
Concentration–response curve of five different reporter genes. Response was determined at tmax of each specific reporter system. NFκB-RE and SBE reporters were stimulated with IL1β (10–8–10–13 g/mL) and TGFβ3 (10–8–10–13 g/mL), respectively. Maximum response was set at 100% and total counts are shown (for all luciferase reporter genes at a concentration of 10–8 g/mL of either IL1β or TGFβ3 and for tdTomato at a concentration of 10–9 g/mL IL1β). Sensitivity is represented as lowest concentration ligand that could be detected significantly compared to control condition. Concentration curve, sensitivity and total counts of (A,B) NFκB-reporters in SW13 cells (C,D) NFκB-reporters in HeLa cells (E,F) SBE-reporters in SW1353 and (G-H) SBE-reporters in HeLa. Data represents mean ± SD of three biological replicates. ns, not significant. N.D. Not Detected.
