Figure 5 | Scientific Reports

Figure 5

From: Reporter gene comparison demonstrates interference of complex body fluids with secreted luciferase activity

Figure 5

Flow chart: recommended applications of the different reporter genes for specific experimental designs. A recombinant protein or small molecule can be studied in both medium as well as cell lysate. Complex body fluids is preferably studied with cell lysates. Unstable nature of NLucP enables higher temporal resolution for monitoring transcriptional activity compared to stable accumulating luciferases (NLuc, FFLuc, GLuc). Furthermore, NLucP exhibits substantial higher inducibility than other luciferases. Among luciferases, FFLuc displays the lowest signal strength.

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