Table 2 Potency and maximum fold induction of NFκB-RE and SBE reporters.

From: Reporter gene comparison demonstrates interference of complex body fluids with secreted luciferase activity

Response element

Reporter gene

pEC50

Fold induction

SW1353

HeLa

SW1353

HeLa

NFκB-RE

NLuc

9.6 ± 0.05

9.0 ± 0.04

37.8 ± 1.09

87.5 ± 1.21

NLucP

9.5 ± 0.08

8.9 ± 0.06

397.7 ± 20.5

1507 ± 57.9

FFLuc

9.8 ± 0.07

9.1 ± 0.03

7.0 ± 0.33

41.1 ± 0.65

GLuc

9.7 ± 0.09

9.5 ± 0.08

27.0 ± 1.49

95.2 ± 5.31

tdTomato

10.1 ± 0.22

9.9 ± 0.11

2.1 ± 0.63

29.7 ± 2.54

SBE

NLuc

9.0 ± 0.12

8.8 ± 0.28

8.1 ± 0.73

1.1 ± 0.42

NLucP

9.2 ± 0.07

9.0 ± 0.06

63.9 ± 3.05

5.2 ± 0.14

FFLuc

9.4 ± 0.08

9.60 ± 0.14

6.5 ± 0.32

1.6 ± 0.13

GLuc

9.4 ± 0.16

10.0 ± 0.13

20.2 ± 2.57

2.8 ± 0.20

tdTomato

N.D

N.D

0.9 ± 1.06

1.0 ± 0.96

  1. Potency (pEC50) and maximum fold induction were determined from a concentration–response experiment at tmax of each specific reporter system. NFκB-RE and SBE reporters were stimulated with IL1β (10–8–10–13 g/mL) and TGFβ3 (10–8–10–13 g/mL), respectively. The presented values were calculated with Graphpad Prism analysis using nonlinear regression: Log(Agonist) vs Response. Data represents mean ± SD of three biological replicates. N.D. Not determined.
Back to article page