Figure 7

Principle component and subsequent expression analysis (volcano plot) of the male and female transcripts controlled for by day to identify differentially expressed genes over time in the male and female transcriptome. The scatter plot displays the largest variance between unfed male and female ticks (Reve Fem Day 0 and Reve Male Day 0) (31.29%) followed by the second largest variance between feeding time points of males (25.2%). The volcano plot represents the differentially expressed female and male transcripts over the feeding period as a fold change against absolute confidence. Differentially expressed transcripts were double filtered to have FDR value < 0.05 and a log2 fold change larger than 4 and smaller than − 4 (dots in blue). Those that are highly up- or downregulated are further to the right and left sides, while highly significant changes appear higher on the plot.