Figure 8
From: Isolation and purification of glycoglycerolipids to induce apoptosis in breast cancer cells
In vitro apoptosis induction by MGDG extracted from the Synechocystis sp. and MGDG standard after 48 and 72 h treatments in (a) BT-474, and (b) MDA-MB-231 cells. The green fluorescence of apoptotic caspase-3/7 proteins increases in both BT-474 and MDA-MB-231 cells treated with MGDG extracted from the Synechocystis sp. The combined phase contrast and the fluorescence images show that the cyanobacterial extracted MGDG enhances the intensity and density of green fluorescence cells indicative of caspase-dependent apoptosis in both cell lines. In contrast, cells treated with MGDG standard molecule did not show any sign of apoptosis in either cell line even after 72 h of treatment. The scale bars represent 100 μm. Quantification of the active caspase-3/7 expression in (c) BT-474, and (d) MDA-MB-231 cells treated with MGDG extracted from the Synechocystis sp. and MGDG standard after 48 h and 72 h of treatments. In BT-474 cells, the active caspase-3/7 expression was increased by 1.7 (p < 0.001) and 3 (p < 0.001) fold after 48 h and 72 h treatments, respectively compared to untreated control. The increase in caspase-3/7 expression was 2.1 (p < 0.001) fold after 72 h treatment in MDA-MB-231 cells compared to the untreated control. Mean ± standard deviation from two independent experiments in at least three replicates (n = 6) are presented. *** indicates the p value < 0.001, showing a statistically significant difference compared to untreated control cells.
