Figure 2 | Scientific Reports

Figure 2

From: An in vivo model allowing continuous observation of human vascular formation in the same animal over time

Figure 2

Determination of antibodies that specifically recognize human ECs. (a) Specificity of anti-CD31 antibodies for human ECs was analyzed by flow cytometry. Left panel shows unstained HUVECs (negative control) and right panel shows huCD31-AF488 Ab, moCD31-AF647 Ab double-stained HUVECs. (b) Specificity of anti-CD31 antibodies for mouse ECs (MS-1 cells) was analyzed by flow cytometry. Left panel shows unstained MS-1, and right panel shows huCD31-AF488 Ab, moCD31-AF647 Ab double-stained MS-1 cells. (c) Representative images of colorectal tumor tissues stained with huCD31-AF488 Ab (green). Nuclei (blue) were labeled with TO-PRO-3. Histograms and images are representative of three independent experiments.

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