Figure 2 | Scientific Reports

Figure 2

From: Role of circulating endothelial cells in assessing the severity of systemic sclerosis and predicting its clinical worsening

Figure 2

Flow cytometry characterization of Circulating Endothelial Cells (CEC). Live nucleated lympho-monocytes were assessed for CD45 and CD34 expression (sky-blue dots in plots A, panel and control). The population defined as positive for CD34 and negative for CD45 expression was gated (blue dots), and evaluated for CD146 positivity. CD146 was gated either versus CD45 (plot B panel) and versus CD34 (plot C panel). Real CD34 bright, CD146 positive events (pink dots) resulted from the intersection of gates displayed in plot A, B and C are CECs. The position of these gates was defined on the basis of matched isotype controls (plot B and C control).

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