Figure 3

LECT2 changed the percentage of M1-like macrophages and M2-like macrophages in the liver of C57BL6 mice and enhanced LPS signaling in KUP5. (A) Flow cytometry analyses of the liver from wild-type and Lect2 KO mice fed an HFD for 4 weeks. The representative plot demonstrates macrophage populations in the liver of Lect2 KO mice fed an HFD. CD11c^high/CD206^low and CD11c^low CD206^high populations were defined as M1-like and M2-like macrophages, respectively. Graphs show quantitation of Gr1-positive macrophages, F4/80 CD11b-positive macrophages, percentages of M1-like macrophages, and percentages of M2-like macrophages (n = 7, 10). B: Western blot analysis of LPS (400 ng/mL)-stimulated JNK, ERK and p38 phosphorylation in KUP5 treated with LECT2 (400 ng/mL). The cells were treated with LPS and LECT2 protein for 30 min (n = 3). (C) Western blot analysis of LPS (400 ng/mL)-stimulated MKK4 and TAB2 phosphorylation in KUP5 treated with LECT2 (400 ng/mL). The cells were treated with LPS and LECT2 protein for 30 min (n = 3). *P < 0.05.