Table 2 Input parameters for each method employed to predict amyloidogenic regions within 3F12E7 scFv amino acid sequence.

From: Generation and functional characterization of a single-chain variable fragment (scFv) of the anti-FGF2 3F12E7 monoclonal antibody

Method

Input parameters

Aggrescan

Not required

Aggrescan3D

Distance of aggregation: 8 Å

Waltz

Threshold: best overall performance

pH: 7

FoldAmyloid

Scale: expected number of contacts 8 Å

Averaging frame: 5

Threshold: 21.4

Tango

N-terminus protected: No

C-terminus protection: No

pH: 7

Temperature: 298.15 K

Ionic strength: 0.02

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