Figure 2 | Scientific Reports

Figure 2

From: The molecular mechanisms of the long noncoding RNA SBF2-AS1 in regulating the proliferation of oesophageal squamous cell carcinoma

Figure 2

SBF2-AS1 promotes the proliferation of oesophageal squamous cell carcinoma cell lines (ECA109 and TE-13 cells). (a) SBF2-AS1 overexpression plasmid, a negative control, SI-SBF2-AS1 and SI-NC were transiently transfected into ECA109 and TE-13 cells, and the expression of SBF2-AS1 was analysed by RT-PCR. (b) Analysis of cell proliferation. The SBF2-AS1 overexpression plasmid, a negative control, SI-SBF2-AS1 and SI-NC were transiently transfected into ECA109 and TE-13 cells, and cell proliferation was detected by RTCA. (c) Analysis of cell proliferation. The SBF2-AS1 overexpression plasmid, a negative control, SI-SBF2-AS1 and SI-NC were transiently transfected into ECA109 and TE-13 cells, and cell proliferation was detected by EdU assay. (d) Colony formation assay. The SBF2-AS1 overexpression plasmid, a negative control, SI-SBF2-AS1 and SI-NC were transiently transfected into ECA109 and TE-13 cells, and colony formation was analysed by colony formation assay. (e) Cell cycle analysis. The SBF2-AS1 overexpression plasmid, a negative control, SI-SBF2-AS1 and SI-NC were transiently transfected into ECA109 and TE-13 cells, and the proportion of cells at each stage of the cell cycle was analysed. (f) Apoptosis analysis. The SBF2-AS1 overexpression plasmid and negative control were transiently transfected into ECA109 and TE-13 cells, the apoptosis rate of the cells was analysed. Data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.01.

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