Figure 3 | Scientific Reports

Figure 3

From: The molecular mechanisms of the long noncoding RNA SBF2-AS1 in regulating the proliferation of oesophageal squamous cell carcinoma

Figure 3

SBF2-AS1 is a sponge RNA for miR-338-3P and miR-362-3P. (a) Localization of SBF2-AS1 in oesophageal squamous cell carcinoma. (b) The expression of miR-338-3P and miR-362-3P in oesophageal squamous cell carcinoma cells and normal oesophageal cells was detected by RT-PCR. (c) A RIP assay using an antibody specifically targeting Ago2 showed that SBF2-AS1 could bind the Ago2 protein in ECA109 cells. (d) RNA-pull down assay. Biotin-miR-338-3P, biotin-miR-362-3P and biotin-miR-NC were transiently transfected into ECA109 cells, and enrichment in SBF2-AS1 was determined and compared. Data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.01.

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