Figure 5

SBF2-AS1 in combination with miR-338-3P and miR-362-3P can increase the expression of E2F1 to promote the proliferation of oesophageal squamous cell carcinoma. (a) Western blot assay (samples derived from another experiment and the blots were processed in parallel). The SBF2-AS1 overexpression plasmid, a negative control, SI-SBF2-AS1 and SI-NC were transiently transfected into ECA109 and TE-13 cells, which were subjected to Western blot analysis of E2F1, Cyclind1 and P21 protein expression. (b) Western blot assay (samples derived for another experiment and the blots were processed in parallel). The SBF2-AS1 overexpression plasmid, a negative control, the SBF2-AS1 overexpression plasmid + miR-338-3P, and the SBF2-AS1 overexpression plasmid + miR-362-3P were transiently transfected into ECA109 and TE-13 cells, which were subjected to Western blot analysis of E2F1, Cyclind1 and P21 protein expression. (c) Colony formation assay. The SBF2-AS1 overexpression plasmid, a negative control, the SBF2-AS1 overexpression plasmid + miR-338-3P, and the SBF2-AS1 overexpression plasmid + miR-362-3P were transiently transfected into ECA109 and TE-13 cells, and colony formation ability was analysed by colony formation assay. (d) Analysis of cell proliferation. The SBF2-AS1 overexpression plasmid, a negative control, the SBF2-AS1 overexpression plasmid + miR-338-3P, and the SBF2-AS1 overexpression plasmid + miR-362-3P were transiently transfected into ECA109 and TE-13 cells, and cell proliferation was detected by EdU assay. (e) Analysis of cell proliferation. The SBF2-AS1 overexpression plasmid, a negative control, the SBF2-AS1 overexpression plasmid + miR-338-3P, and the SBF2-AS1 overexpression plasmid + miR-362-3P were transiently transfected into ECA109 and TE-13 cells, and the expression of SBF2-AS1 was analysed by RT-PCR. (f) Analysis of cell proliferation. The SBF2-AS1 overexpression plasmid, a negative control, the SBF2-AS1 overexpression plasmid + miR-338-3P, and the SBF2-AS1 overexpression plasmid + miR-362-3P were transiently transfected into ECA109 and TE-13 cells, and cell proliferation was detected by RTCA. Data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.01.