Figure 6
METH treatment activates proline synthetic pathway in cortical regions of mice brain-Mice were injected intra-peritoneally (IP) with a single dose of METH (2 mg/kg) for acute METH treatment. Then the animals were euthanized after 24 h and whole brain was isolated from METH treated (n = 4) and saline-treated (n = 4) animals. Cortical regions were then sliced and homogenized for measuring the levels of proline synthetic enzymes. (A) Representative western blot probing P5CS levels in the cortices of saline treated mice and METH treated mice with β-actin used as a loading control (n = 3). (B) Quantitative representation of P5CS western blot of either saline or METH treated mice. (C) Western blot probing for PYCR2 in the cortical region of saline treated mice and METH treated mice with β-actin used as a loading control (n = 3). (D), Quantitative representation of PYCR2 western blot of either saline or METH treated mice. Data are presented as the mean ± SEM of at least three independent experiments. *p < 0.05 represents statistical comparison of untreated vs METH-treated samples.
