Figure 6
VASH1 protein transfer increases the cavernous expression of angiogenic factors in diabetic mice. (a,b) Angiopoietin-1 (Ang1, green) and PECAM1 (red), or vascular endothelial growth factor (VEGF, green) and PECAM-1 (red) staining in cavernous tissue from age-matched control and streptozotocin-induced diabetic mice 2 weeks after receiving repeated intracavernous injections of PBS (days − 3 and 0) or VASH1 protein (days − 3 and 0; 4 μg/20 μL). Scale bar = 100 µm. (c,d) Ang1 and VEGF-immunopositive areas were quantified by Image J. Each bar depicts the mean (± SE) values from N = 6 animals per group. *P < 0.05, **P < 0.01 vs. control group. #P < 0.05, ##P < 0.01 vs. PBS-treated DM group. (e,f) Representative Western blots for Ang1 and VEGF in each group of mice. (g,h) The relative ratio of Ang1 and VEGF compared with that of β-actin. Each bar depicts the mean (± SE) values from N = 4 animals per group. **P < 0.01 vs. control group. ##P < 0.01 vs. PBS-treated DM group. DM diabetes mellitus. Full length blot as well as the related images available in the supplementary data.
