Figure 2 | Scientific Reports

Figure 2

From: CD20 positive CD8 T cells are a unique and transcriptionally-distinct subset of T cells with distinct transmigration properties

Figure 2

RNAseq analysis of CD20 + effector memory T cells reveals an altered transmigration and adhesive profile combined with an enhanced activation status. (A) Pie chart displaying FACS analysis of CD20 + T cells in a effector memory lymphocytes subset. (B) MA plot displaying DEGs in CD20 + CD3 + CD8 + CD45RO + CCR7- versus CD20- CD3 + CD8 + CD45RO + CCR7- with significantly upregulated genes in blue and significantly downregulated genes in red. DEGs were determined by DESeq2 (Wald test and corrected for multiple testing using the Benjamini and Hochberg method). Genes with a Benjamini–Hochberg FDR < 0.05 and log2FoldChange ≥ 2 were selected for further analysis. (C) log2FoldChange of a subset of significantly DEGs, with standard error (L2fcSE), with activation/memory/cytotoxic/migratory/adhesive backgrounds. (D) Search of DEGs for enriched terms using the tissue expression library (GNF U133A quartile) of the Database for Annotation, Visualization and Integrated Discovery (DAVID), (E) using the Jensen TISSUES library of Enrichr (P-value ranking) and (F) using the KEGG 2019 human pahtway of Enrichr (P-value ranking).

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