Figure 5

CD20 + T lymphocytes engraft, cause X-GvHD in RAG2-/-γc-/- mice and display altered adhesive pattern in mouse spleens. (A) 15 × 10^6 fresh huPBMCs (n = 5), 40 × 10^6 CD3/CD28 stimulated cultured primary T cells (n = 10) and 40 × 10^6 CD3/CD28 stimulated CD20-transduced primary T cells (n = 10) from the same donor were injected into irradiated and macrophage-depleted mice. The non-transduced and transduced cells were cultured for 6 days. Peripheral blood was collected once a week and the percentage of human CD45 and (B) CD3/CD20 + cells was determined. (C) The capability of the T cells to induce GvHD was determined by weight loss, reduced mobility of the mice and development of ruffled fur. These criteria were scored as arbitrary units (AU) and set against time. The mean ± SD are shown. 2way ANOVA’s were performed to deterime significance. (D) Immunohistochemical evaluation was performed in mice injected with T cells ectopically expressing CD20. Infiltration of human CD3 + cells is shown in the left columns and the infiltration of CD20 + cells into the organs is shown in the right columns. These figures show the colon A, the lung B, the liver C, the spleen D and spleen E (injection of non-transduced human PBMCs, anti-CD20 B cell stain).