Figure 3

HMPV-specific TCD8 peak in the lung on day 13 in B7tg mice. B7tg mice were infected i.n. with 1 × 106 PFU HMPV and lungs and spleens collected on days 5, 7, 10, 13, 16, and 22 post-infection. Live/dead dye and fluorescently labeled CD8 and CD3 antibodies were used to identify live CD8+ T lymphocytes and fluorescently labeled HMPV-specific tetramers used to enumerate epitope-specific TCD8. Data are representative of two independent experiments with N = 3–5 mice per experiment.