Figure 4 | Scientific Reports

Figure 4

From: Novel HLA-B7-restricted human metapneumovirus epitopes enhance viral clearance in mice and are recognized by human CD8+ T cells

Figure 4

Epitope vaccination with HMPV peptide reduces lung virus titer in B7tg mice. (A) B7tg mice were vaccinated with LPS-matured bone marrow-derived DCs loaded with vaccinia virus (VV) or individual HMPV peptides, allowed to rest for 2 weeks, and then infected i.n. with 1 × 106 PFU HMPV. (B) B7tg mice were vaccinated with combined HMPV peptides plus LPS, allowed to rest for 4 weeks, and then infected i.n. with 1 × 106 PFU HMPV. In both experiments, lungs were collected on day 5 post-infection and HMPV titer determined using plaque assay. Error bars represent SEM. *P < 0.05, **P < 0.01, ***P < 0.001 compared to mock, one-way Anova with Dunnet’s correction for multiple comparisons. Dotted line indicates limit of detection.

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