Figure 9

Histopathological study of the liver. (a) Photomicrograph of control group displaying liver tissue with normal hepatocytic cords (Black arrows) crowded around a clear central vein (CV) (H&E stains, X400). (b) Photomicrograph of HFD/STZ-induced shows a marked dilated hemorrhage portal tract, bile duct (Black arrow), dilated hemorrhage central vein (Blue arrow), and edema. Besides, few infiltrating lymphocytes (Red arrow), and dilation of the sinusoid were seen (vacuolar and hydropic degeneration), which indicate the fatty degeneration of the liver (Green arrows) (H&E stain, X400). (c) Photomicrograph of HFD/STZ + Cur shows crowded hepatocyte around the congested portal tract (Black arrow) with few fibrotic cells (Red arrow). Most hepatocytes with rounded nuclei and vacuolated cytoplasm (Green arrows). Many karyolysis hepatocytes and necrotic one was seen (Blue arrow) (H&E stains, X400). (d) Photomicrograph of HFD/STZ + CurNPs-10 shows a mild dilated portal tract (Black arrow), the hepatocytes are crowded and have small dark nuclei (Green arrows) (H&E stains, X400). (e) Photomicrograph of HFD/STZ + CurNPs-50 shows the necrotic changes of hepatocytes (dark condensed/pyknotic nucleus) (Green arrows) (H&E stains, X400). (f) Photomicrograph of HFD/STZ + ZnSO₄ shows marked dilated portal tract (Black arrow) surrounded with few fibrotic cells (Red arrow), dilated bile duct, and crowded hepatocytes were appeared with round nuclei and vacuolated cytoplasm (Green arrows). With a few necrotic and pyknotic one was seen (Blue arrow) (H&E stains, X400). (g) Photomicrograph HFD/STZ + ZnONPs-10 shows marked dilated three portal tracts and bile with the absence of the fibrotic cells (Black arrows). Reorganized hepatocytes architecture with dark rounded nuclei with homogenous non-vacuolated cytoplasm was seen (Green arrows). Few necrotic hepatocytes (Blue arrows) and mildly dilated sinusoid with Kuffer's cell were seen (Red arrows) (H&E stains, X400). (h) Photomicrograph of HFD/STZ + ZnONPs-50 shows marked recovering of hepatocytes were appeared with round nuclei containing prominent nucleoli and homogenous non-vacuolated cytoplasm (Black arrows). Few necrotic hepatocyte and pyknotic one was seen (Blue arrows) (H&E stains, X400). (i) Photomicrograph of HFD/STZ + MT shows marked dilated and congested portal tract and bile duct surrounded with fibrotic cells (Black arrows). Proliferating hepatocytes were appeared with round nuclei and vacuolated cytoplasm (Blue arrows) (H&E stains, X400).