Figure 4

Negative images of electrophoresis gels showing the results of the (A) D-loop/sdY and (B) clock1a/sdY PCR sex identification assays for six of the analyzed Pacific salmon (Oncorhynchus spp.) samples (BIS#). The approximate positions of the internal positive control (D-loop and clock1a) and sdY amplicons are indicated by the labelled arrows. BK denotes the blank extraction control processed alongside the samples. NEG denotes negative PCR controls. The 100 bp ladder is from Invitrogen (Vilnius, LT). PCR products were pre-stained with SYBR Green I (Invitrogen, Eugene, OR, USA). Unprocessed images of the electrophoresis gels are presented in Supplementary Fig. 1 and Supplementary Fig. 2.