Figure 5

The gating strategy for the Annexin-V/PI apoptosis analysis. CD34⁺ cells were gated on forward (FSC) versus side scatter (SSC) to select the cell population (A and D). Next, the cells were gated on FSC-Height (FSC-H) vs FSC-Area (FSC-A) to exclude all the doublets and to generate the singlets gate (B and E). Finally, all the subpopulations were analysed on the Annexin V-FITC versus PI scatter for live, early and late apoptosis at 18 h post-irradiation (C and F). The upper part (A–C) represents the gating strategy of CD34⁺ cells irradiated with a low dose of neutrons at 0.5 Gy; and the lower part (D–F) CD34⁺ cells irradiated with a high dose at 3 Gy which resulted in a higher percentage of late apoptosis.