Table 1 Parameters separated by category.

From: Modeling characterization of the vertical and temporal variability of environmental DNA in the mesopelagic ocean

Category

Parameter in model

Constant value used in model

Changes in sensitivity test?

Changes in percent migrate test?

Notes

Organism/eDNA

eDNA shedding rate

1 eDNA mass unit/time step

No

No

Still relatively unknown, likely varies with time (many scales from second to years) and by organism, maybe varies with temperature or other biotic/abiotic conditions

Organism/Ocean

Day depth

500 m

No

No

Varies with light/temperature, Varies with organism

Organism/Ocean

Night depth

50 m

No

No

Varies with light/temperature/food availability, varies with organism

Organism/Ocean

Migration times

Up: 2 h before sunset to 1 h after sunset; down: 1 h before sunrise to 2 h after sunrise; sunrise/sunset: determined by season

No

No

Varies with light/temperature/food availability, varies with organism

Organism

Thickness of “layer”

20 m

No

No

Varies with organism

Organism

Percent of individuals (within a species) that vertically migrate

N/A

Yes - 0, 50, 100%

Yes - 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100%

Still relatively unknown

Organism/eDNA

eDNA size distribution

N/A

Yes - ratio of LP:SP - 1:1, 1:2, 2:1

No

Unknown, likely varies with time

eDNA

Settling rate

N/A

Yes - 0, 25, 50 m/day

No

Unknown, likely varies with eDNA form (use value based on literature for marine snow)

eDNA

Breakdown rate

0.19 1/h

No

No

Unknown (use value based on literature for fecal pellet)

eDNA/Ocean

Decay rate

N/A

Yes - 0.01, 0.1 1/h and k = 0.05 + 0.0014*T 1/h

No

Varies with temperature (possibly other biotic/abiotic factors and by organism) (Supplemental Fig. S3)

Ocean

Mixing profile

Determined by season

No

No

Varies by season and depth (Supplemental Fig. S1)

Ocean

Vertical advection profile

N/A

Yes - 0, 1E–4, −1E–4 m/s

No

Varies by season and depth (Supplemental Fig. S2)