Figure 1

Schematic overview of urine storage conditions. (A) Schematic overview of the two storage methods of urine from seven different donors. Urine was stored either at 4 °C or with EDTA at RT for up to 8 days. EV characterization was performed on all seven donors using EM and EVQuant. Urinary EVs were isolated from three donors and proteomes were measured using DIA-LC–MS/MS. (B) EM pictures of EVs directly after urine collection, or after 8 days of storage using the two different storage conditions. (C) Effect of storage time and protocol on the concentration of EVs as measured using the EVQuant assay.