Figure 4
Effect of EDTA on urinary EV proteins. (A) Effect of EDTA on the concentration of EVs as measured using the EVQuant assay. (B) Data presence plot, showing a high data presence amongst all samples. The proteins were ranked according to data presence and average log2-intensity. The missing values are gray. (C) Venn diagram showing the number of proteins identified in urine samples stored at 4 °C vs EDTA. (D) Protein rank plot showing that the 98 unique proteins identified upon addition of EDTA (indicated in blue) are low abundant. (E) Number of proteins identified in the three donors with and without EDTA addition, showing no significant difference between the three donors. (F) Pierson correlation plot showing a high correlation between all urine samples. Blue bars are samples where EDTA is added, pink bars indicate the samples stored at 4 °C. (G) Protein rank plot showing that the proteins identified and EV markers are highly consistent. (H) Boxplots of known EV markers showing no difference in abundancy between the two storage methods.
